| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 131 | Burkholderia mallei | GGCTTCGCGACGACATGTA | ACTTGCCGTCGAACGTCA | 60.52 | 59.59 | 258 | 43 |
100.00%
|
100.00%
|
| Primer 132 | Burkholderia mallei | GCTTCGCGACGACATGTAC | ACTTGCCGTCGAACGTCA | 59.38 | 59.59 | 257 | 43 |
100.00%
|
100.00%
|
| Primer 133 | Burkholderia mallei | ATGCGTGCGTCGACTACA | ACACGATGAAGCCCTGCA | 59.44 | 59.25 | 246 | 42 |
100.00%
|
100.00%
|
| Primer 134 | Burkholderia mallei | ACATGTACGCACTCGACGC | AGAAGTTCGGCAGCGCAA | 60.80 | 60.28 | 279 | 41 |
100.00%
|
100.00%
|
| Primer 135 | Burkholderia mallei | TGCGGTTGTCGACCTGAA | AACTGATCGTCGGTGAGCG | 59.18 | 60.15 | 288 | 40 |
100.00%
|
100.00%
|
| Primer 136 | Burkholderia mallei | TCGTTCACTGCGCTCGAT | TGAGCTGATCCTTGCGCT | 59.43 | 59.02 | 251 | 39 |
100.00%
|
100.00%
|
| Primer 137 | Burkholderia mallei | TGCGGTTGTCGACCTGAA | TGCCTGAACTGATCGTCGG | 59.18 | 59.78 | 294 | 39 |
100.00%
|
100.00%
|
| Primer 138 | Burkholderia mallei | TGCGGTTGTCGACCTGAA | GCCTGAACTGATCGTCGGT | 59.18 | 59.78 | 293 | 39 |
100.00%
|
100.00%
|
| Primer 139 | Burkholderia mallei | AGCGCAATCAGCCGATGA | GATCCACCTCGACTTGCGT | 59.81 | 59.78 | 294 | 38 |
100.00%
|
100.00%
|
| Primer 140 | Burkholderia mallei | AGCGCAATCAGCCGATGA | ATCCACCTCGACTTGCGT | 59.81 | 58.62 | 293 | 38 |
100.00%
|
100.00%
|